To test the affinity between protein A and multiple small molecules.
Technical difficulties：
▇The wild-type protein A tends to precipitate upon addition of high
concentrations of reducing agents, rendering it unsuitable for SPR testing.
▇The protein gradually loses activity during the testing process.
Solution：
▇By analyzing the protein sequence, truncating the protein, and introducing
mutations to surface cysteine residues, a stable protein was obtained.
▇Initially, protein A was immobilized on the chip via amino coupling, but
during the testing process, the protein gradually lost activity. Subsequently,
protein capture was achieved using anti-His antibodies to immobilize the
protein on the chip, ensuring its activity throughout the testing process.
Results：
23 Feb 
