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Actinomycetes-derived imine reductases with a preference towards bulky amine substrates
Commun Chem. 2022 Oct 8;5(1):123. doi: 10.1038/s42004-022-00743-y.
This paper reported the imine reductase IR-G02, which is capable of accepting bulky amine substrates.
ReadCrystal contribution: Structural biology related work of IR-G02 such as structure determination.
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Atomic-resolution structures from polycrystalline covalent organic frameworks with enhanced cryo-cRED
Nat Commun. 2022 Jul 11;13(1):4016. doi: 10.1038/s41467-022-31524-9.
In this paper, a method is established to determine the structure of polycrystalline 3D COF at atomic level using enhanced cryo-continuous rotation electron diffraction (cryo-cRED).
This method was proposed by the team of Prof. Junliang Sun, the founder of ReadCrystal.
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Studies on the Crystal Forms of Istradefylline: Structure, Solubility, and Dissolution Profile
Crystals. 2022, 12(7), 917; https://doi.org/10.3390/cryst12070917
Istradefylline as a selective adenosine A2A-receptor antagonist is clinically used to treat Parkinson’s disease. In this paper, its crystal forms were studied.
ReadCrystal contribution: study of crystal form, such as crystal preparation and structure determination.
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Design and Synthesis of a Zeolitic Organic Framework
Angew Chem Int Ed Engl . 2022 Jun 13;61(24):e202203584. doi: 10.1002/anie.202203584.
In this paper, the first example of a 3D covalent organic framework with zeolitic network, namely the zeolitic organic framework (ZOF), ZOF-1 was reported. Its structure was determined by MicroED technique.
Structure determination of MicroED in this paper was done by the team of Prof. Junliang Sun, the founder of ReadCrystal.
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Tuning an Imine Reductase for the Asymmetric Synthesis of Azacycloalkylamines by Concise Structure-Guided Engineering
Angew Chem Int Ed Engl . 2022 Jun 13;61(24):e202201908. doi: 10.1002/anie.202201908. Epub 2022 Apr 7.
In this paper, a concise strategy combining rational cavity design, combinatorial active-site saturation test (CAST), and thermostability engineering was designed to transformed the weakly active imine reductase IR-G36 into a variant M5 with superior performance.
ReadCrystal contribution: Structural biology related work of IR-G35 and M5 mutation such as protein expression and structure determination.
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Structural origin of the high-voltage instability of lithium cobalt oxide
Nat Nanotechnol . 2021 May;16(5):599-605. doi: 10.1038/s41565-021-00855-x.
This paper reported structural differences at the atomistic level between two commercial LCO materials: a normal LCO (N-LCO) and a H-LCO.
Structure determination of LCO was done by the team of Prof. Junliang Sun, the founder of ReadCrystal. Three-dimensional continuous rotation electron diffraction method was used to determine the structure, also called MicroED.
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Atomically precise single-crystal structures of electrically conducting 2D metal-organic frameworks
Nat Mater . 2021 Feb;20(2):222-228. doi: 10.1038/s41563-020-00847-7.
This paper reported crystal structures of 2D π-conjugated MOFs with electrical conductivity, and the atomic-level resolution structures were precisely determined by the cultivation of large single crystals and MicroED techniques.
Structure determination of MicroED in this paper was done by the team of Prof. Junliang Sun, the founder of ReadCrystal.
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FAD-dependent enzyme-catalysed intermolecular [4+2] cycloaddition in natural product biosynthesis
Nat Chem. 2020 Jul;12(7):620-628. doi: 10.1038/s41557-020-0467-7.
This paper reported the key steps of active natural product biosynthesis in the traditional Chinese medicine, Moraceae (mulberry), and reported the first monofunctional enzyme catalyzing the intermolecular Diels-Alder reaction existing in nature.
This paper was published in collaboration with the team of Prof. Xiaoguang Lei, the founder of ReadCrystal.
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Structural basis of Gs and Gi recognition by the human glucagon receptor
Science . 2020 Mar 20;367(6484):1346-1352. doi: 10.1126/science.aaz5346.
This paper reported the structures of the human glucagon receptor (GCGR) bound to glucagon and distinct classes of heterotrimeric G proteins, Gs or Gi1 which were determined by Cryo-EM SPA.
The researcher who was responsible for the structure determination of GCGR by Cryo-EM SPA in this paper is currently working at ReadCrystal, in charge of the Cryo-EM SPA project.
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Single crystal of a one-dimensional metallo-covalent organic framework
Nat Commun . 2020 Mar 18;11(1):1434. doi: 10.1038/s41467-020-15281-1.
In this paper, a single crystal of mCOF was synthesized and its structure was determined by MicroED technique.
Structure determination of MicroED in this paper was done by the team of Prof. Junliang Sun, the founder of ReadCrystal.
