Publications|ReadCrystal-Your Exclusive Expert In Structure Determination-Structural Biology-X-Ray Crystallography-CryoEM-SPA-MicroED-Drug Discovery

FAD-dependent enzyme-catalysed intermolecular [4+2] cycloaddition in natural product biosynthesis

Nat Chem. 2020 Jul;12(7):620-628. doi: 10.1038/s41557-020-0467-7.

This paper reported the key steps of active natural product biosynthesis in the traditional Chinese medicine, Moraceae (mulberry), and reported the first monofunctional enzyme catalyzing the intermolecular Diels-Alder reaction existing in nature.

This paper was published in collaboration with the team of Prof. Xiaoguang Lei, the founder of ReadCrystal.

Determination of the Absolute Configuration of 2′-Amino-3′-methylacetophenone Based on the Modified Mosher and Microcrystal Electron Diffraction Methods

Chem. Pharm. Bull. 2025 Jun 10;73(6):520-525. doi: 10.1248/cpb.c25-00284.

This study presents the determination of the absolute configuration of a chiral aromatic alcohol derivative by combining the modified Mosher method with Microcrystal Electron Diffraction (MicroED) techniques.

ReadCrystal was responsible for determining the absolute configuration of the chiral aromatic alcohol derivative (-)-2. Utilizing our proprietary MicroED technology platform, we successfully collected and solved the microcrystal electron diffraction data, thereby directly determining its absolute stereochemistry.

Comprehensive analysis of Enterobacteriaceae IncX plasmids reveals robust conjugation regulators PrfaH, H-NS, and conjugation-fitness tradeoff

Commun Biol . 2025 Mar 4;8(1):363. doi: 10.1038/s42003-025-07782-w.

This study comprehensively analyzed IncX plasmids in Enterobacteriaceae, revealing that the plasmid-encoded activator PrfaH and inhibitor H-NS are key factors regulating their conjugative transfer and indicating that PrfaH may be a potential target for combating antibiotic resistance.

ReadCrystal's contribution: resolve the crystal structure of PrfaH, identifying the key residues mediating target interactions, and providing a structural basis.

Engineered Metal-Organic Framework with Stereotactic Anchoring and Spatial Separation of Porphyrins for Amplified Ultrasound-Mediated Pyroptosis and Cancer Immunotherapy

Angew Chem Int Ed Engl . 2025 Mar 3;64(10):e202421402. doi: 10.1002/anie.202421402. Epub 2024 Nov 28.

This study designed a porphyrin-based metal-organic framework, Fe(TCPP)-MOF, which achieves stereospecific anchoring and spatial separation of porphyrins through a dual-solvent strategy. This avoids aggregation-induced quenching, enhances interaction with oxygen, generates a large amount of singlet oxygen under ultrasound activation, and accelerates the Fenton reaction to produce hydroxyl radicals.

ReadCrystal's contribution：assist in analyzing the regular rigid conformation of Fe(TCPP)-MOF through MicroED analysis.

Unconventional Heterobidentate Coordination of 4-Hydroxypyridine Leading to Remarkably Strong Second-Harmonic Generation in Zn(C5H4NO)2

Angew Chem Int Ed Engl . 2025 Feb 24;64(9):e202420810. doi: 10.1002/anie.202420810. Epub 2024 Dec 9.

This study reports that in the novel compound Zn(C₅H₄NO)₂, the unconventional heterobidentate coordination of 4-hydroxypyridine ligands with Zn²⁺ leads to uniform polarization alignment of [ZnO₂N₂] tetrahedral units, resulting in a remarkably strong second-harmonic generation, along with suitable optical band gap, high thermal stability, excellent air and water resistance, and photoluminescence with enhanced lifetime;

ReadCrystal's contribution: provide structural insights through MicroED

Ultrahigh–surface area covalent organic frameworks for methane adsorption

Science 2024, 386, 693-696. doi: 10.1126/science.adr0936.

This study reports the use of continuous rotation electron diffraction to determine the structure of a three-dimensional covalent organic framework with an interpenetrated topology, and investigates its ultrahigh specific surface area and methane adsorption performance.

ReadCrystal was responsible for the structure determination of the COF material. Using our MicroED technology platform, we successfully solved the crystal structure of the three-dimensional covalent organic framework with an interpenetrated topology.